Chapter 2

Assays of Peroxidase Activity

Bernardo Dias Ribeiro and Maria Alice Zarur Coelho

Abstract

Peroxidases are enzymes that use various peroxides as electron acceptors in oxidation, and are widespread in nature. Their activities are usually determined by spectrophotometric methods, which a condensation or oxidation product is formed in the presence of H2O2, or by titrimetric methods, using potassium permanganate or sodium thiosulfate, which measure free hydrogen peroxide in solution.

Total Pages: 27-38 (12)

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