Chapter 11

Qualitative and Quantitative to Determine Peptidase Activity

Alane Beatriz Vermelho, Edilma Paraguai de Souza, Daniel Paiva and Giseli Capaci Rodrigues

Abstract

The aim of this chapter is to present the modern analytical methodologies used to study peptidases, such as the colorimetric methods and spectrophotometric analyses. A general method for the detection of peptidases activity includes gelatin and other substrates incorporated into SDS-PAGE using a technique known as zymography. All methodologies presented here are important tools for the discovery and characterization of peptidases.

Total Pages: 262-280 (19)

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