Calcium Signaling and Angiogenesis
- Pp. 179-201 (23)Luca Munaron
Proliferation and migration of vascular endothelial cells (ECs) are critical steps in angiogenesis and are strictly controlled by a number extracellular stimula. Proangiogenic peptides binding to tyrosine kinase receptors (i.e. VEGFs and FGFs) are released by several cell types, including ECs and tumor cells. Proangiogenic intracellular signalling cascades involve many messengers working in a sort of network. In particular, in this chapter, we describe the properties and functions of the intracellular calcium signals (Ca<sub>i</sub>), a universal, evolutionary conserved and highly versatile pathway involved in the regulation of EC proliferation and migration. </p><p> Angiogenic factors generate Ca<sub>i</sub> rises via two mechanisms: entry from extracellular medium, through the opening of calcium-permeable channels in the plasmamembrane, or release from intracellular Ca<sup>2+</sup> stores. Calcium entry, the main topic of this chapter, can be dependent on previously InsP3-activated emptying of calcium stores (storeoperated Ca<sup>2+</sup> entry - SOCE), or independent on it (non store-operated Ca<sup>2+</sup> entry - NSOCE). The intracellular pathways underlying endothelial Ca<sup>2+</sup> entry involve, among the other pathways, arachidonic acid (AA) and nitric oxide (NO) metabolism. Even if some Ca<sup>2+</sup> entry blockers are under clinical trial with encouraging results, a better knowledge about the molecular nature of proangiogenic Ca<sup>2+</sup> channels and their intracellular regulation in healthy and pathological processes could lead to new and more powerful strategies in the therapeutical approaches aimed to interfere with altered tissue vascularization. Here, we discuss the state of the art in the field of calcium signaling and angiogenesis, the related recent literature and patents.