From Genomes to Transcriptomes and Secretomes: A Fungal Perspective with Insights into Biorefinery Applications
- Pp. 366-394 (29)Carlaile F.O. Nogueira, Gabriela L. Berto, Gustavo H. Goldman, Rolf A. Prade and Fernando Segato
The post-genomic sequencing stimulated all sorts of biological sequencing strategies such as genomic DNA sequencing, mRNA-Seq, LC-MS/MS peptide counting and metabolomics enabling the establishment of complex and interactive networks made out of genes, proteins and metabolites. High-throughput methodologies analyze multiple metabolic pathways simultaneously offering benefits in the exploitation of new biorefining processes. However, it is evident that a single approach cannot unravel or solve the complexities of fundamental microbial biology by just making the integration of multiple information layers. Therefore, efforts have been made to systematically analyze the correlation between transcriptomic and proteomic datasets, improving the chances of capturing gene protein relationships as well as integration of novel biological processes. Here, we describe high-throughput genomics and proteomics tools, discuss the associated challenges and analyze fungal secretomes.