Evaluation of Cellular processes by In Vitro Assays

by

Taseen Gul

DOI: 10.2174/97816810870301180101
eISBN: 978-1-68108-703-0, 2018
ISBN: 978-1-68108-704-7



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This handbook presents information on different cell culture assays which can be used to perform experimental analysis. Readers are in...[view complete introduction]
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Table of Contents

Foreword

- Pp. i
Khalid Majid Fazili
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Preface

- Pp. ii
Taseen Gul
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Abbreviations

- Pp. iii-iv (2)
Taseen Gul
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Basics of In Vitro Cell Culture

- Pp. 1-19 (19)
Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq
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Methods of Transfection

- Pp. 20-28 (9)
Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq
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Apoptosis

- Pp. 29-43 (15)
Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq
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Cell Cytotoxicity, Viability and Proliferation

- Pp. 44-57 (14)
Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq
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Reactive Oxygen Species (ROS)

- Pp. 58-67 (10)
Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq
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Protein-Protein Interactions

- Pp. 68-75 (8)
Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq
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Protein-Nucleic Acid Interactions

- Pp. 76-84 (9)
Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq
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Glossary

- Pp. 85-87 (3)
Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq
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Subject Index

- Pp. 88-94 (7)
Taseen Gul, Henah Mehraj Balkhi and Ehtishamul Haq
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Foreword

This book provides hands on information about the various in vitro techniques and is preceded by the information about the principles and the basics of the technique. The authors have clearly and concisely discussed several concepts and methods which will be useful for the under-graduate and post-graduate students during their laboratory courses. The manuscript will equally contribute to the day-to-day research work of the scholars.

I strongly recommend publishing of this book and I wish best of luck to all the authors.

Dr. Khalid Majid Fazili
Department of Biotechnology
University of Kashmir,
Hazratbal, Srinagar,
India


Preface

During the course of my doctoral degree, I was very much interested in the methods involved in the understanding effect of drugs in in vitro conditions. The students and scholars often find it confusing to select a particular assay on cell viability, cell proliferation and apoptosis. While teaching at the post-graduate level, I got to know about the problems our students face in understanding the basics of in vitro assays. Keeping in view these facts, I tried to address these concerns by providing a basic introduction about various assays in the form of this book. The book gives comparative analysis of different assays and discusses the advantages and disadvantages related to it. Each experimental protocol is preceded by the information about the principle and the basics of the technique.

I hope my little effort will benefit the students and scholars who are interested to know the basic fundamentals of cellular assays. To make this book better, the criticism and suggestion are most welcomed.

Taseen Gul
Department of Biotechnology,
University of Kashmir,
Hazratbal, Srinagar,
India

List of Contributors

Author(s):
Taseen Gul




Contributor(s):
Taseen Gul
Department of Biotechnology, Science Block
University of Kashmir, Hazratbal
Srinagar-190006


Henah Mehraj Balkhi
Department of Biotechnology, Science Block
University of Kashmir, Hazratbal
Srinagar-190006


Ehtishamul Haq
Department of Biotechnology, Science Block
University of Kashmir, Hazratbal
Srinagar-190006




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