Microsurgery in Liver Research


by

Maria-Angeles Aller , Jaime Arias

DOI: 10.2174/97816080506801090101
eISBN: 978-1-60805-068-0, 2009
ISBN: 978-1-60805-406-0



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Indexed in: Web of Science, Scopus.

During the last two decades microsurgery has progressively taken the place of macrosurgery since it largely improves the experimental ...[view complete introduction]

Table of Contents

Foreword

- Pp. i

Sir Roy York Calne

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Preface

- Pp. ii

Maria-Angeles Aller and Jaime Arias

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Contributors

- Pp. iii

Maria-Angeles Aller and Jaime Arias

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Brief History of Experimental Liver Microsurgery

- Pp. 1-6 (6)

Maria-Angeles Aller and Jaime Arias

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Laboratory Rat Management

- Pp. 7-31 (25)

Maria-Angeles Aller, Maria Paz Nava, Marta Mendez, Patri Vergara and Jorge-Luis Arias

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Microsurgery: Instruments and Techniques

- Pp. 32-48 (17)

Maria Angeles Aller and Jaime Arias

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Anesthesia, Analgesia and Postoperative Care

- Pp. 49-70 (22)

Maria Angeles Aller and Maria Paz Nava

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Extraction Techniques of Blood, Bile and Lymph Samples

- Pp. 71-93 (23)

Maria-Angeles Aller, Maria-Paz Nava, Marta Mendez and Jaime Arias

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Laparotomies

- Pp. 94-98 (5)

Maria-Angeles Aller, Manuel Losada, Marta Mendez and Jaime Arias

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Liver Anatomy

- Pp. 99-107 (9)

Laureano Lorente, Maria-Angeles Aller and Jaime Arias

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Vascular Microanastomoses

- Pp. 108-116 (9)

Maria-Angeles Aller, Fernando Sanchez-Patan and Jaime Arias

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Portal Surgery: Portosystemic Shunts and Portal Hypertension

- Pp. 117-136 (20)

Maria-Angeles Aller, Marta Mendez, Maria-Paz Nava, Laudino Lopez, Diana Curras, Ana de Paz, Jorge-Luis Arias and Jaime Arias

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Extrahepatic Cholestasis

- Pp. 137-156 (20)

Maria Angeles Aller, Isabel Prieto, Arturo Cruz, Manuel Losada, Jose-Ignacio Arias, Jose Garcia-Dominguez, Salvador Argudo, Jorge-Luis Arias and Jaime Arias

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Hepatectomies

- Pp. 157-167 (11)

Maria-Angeles Aller, Laureano Lorente, Isabel Prieto and Jaime Arias

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Orthotopic and Heterotopic Liver Transplantation

- Pp. 168-198 (31)

Lorente Lorente, Maria Angeles Aller and Jaime Arias

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Appendix

- Pp. 199-218 (20)

Maria-Angeles Aller and Jaime Arias

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Foreword

This book is an outline of the achievements of experimental liver microsurgery. Microsurgery has now come of age; starting in the 1950s with optics and surgical instruments, often modified from other uses, the experimental surgeon has now operating microscopes, micro-instruments and sutures of a wide variety and high quality. The authors of this book are to be congratulated in bringing together the current status of microsurgery in experimental liver research. This is an area where there have been and continue to be important advances as a result of the skill of microsurgeons in providing an opportunity to study experimental procedures in a large number of animals. I remember in the 1960s visiting Dr. Sun Lee, who was regarded as the father of microsurgery of the liver. I was impressed with his extraordinary skill, precision and perseverance, and it was under his tutelage that other young surgeons learned the techniques of liver surgery in small animals and, in particular, transplantation of the liver. It is timely that this book has been written, the chapters are clearly laid out with illustrations that are informative and helpful in a practical sense. Nevertheless, as the authors point out, for any of these techniques instead of the surgeon “having a go”, it is far quicker, cheaper and more humane to visit a centre where these techniques are routine and obtain a thorough training before embarking on microsurgical procedures independently. Microsurgery of the liver has contributed important information on liver physiology, immunology and the potential and limitations of surgical procedures. I can thoroughly recommend the book which should be available to all liver surgeons who are interested in advancing their subject.

Sir Roy Calne FRS


Preface

This e-book has been prepared to provide researchers, whether surgeons or not, with a brief overview of the current liver microsurgical techniques in laboratory rats and mice.

Special emphasis is placed on the more useful microsurgical techniques used for obtaining experimental models to study liver pathophysiology, diagnostics and treatments. New procedures have also been added that reflect our current practices. Thus, we wish to express our gratitude to our colleagues who have used and perfected the microsurgical techniques that this e-book describes.

We would like to thank many people whose talents and time made this e-book possible. We acknowledge Maria Elena Vicente for preparing the manuscript, Elizabeth Mascola for translating it into English, and the librarians of the Complutense University Medical School, particularly the Director, Juan Carlos Domínguez Martínez, Subdirector Manuela Crego and María José Valdemoro. This book was supported, in part, by grants from Complutense University of Madrid (AE1/09/17064) and Mutua Madrileña Research Foundation (Ref. nº AP69772009).

Maria-Angeles Aller & Jaime Arias
Complutense University of Madrid
Spain

List of Contributors

Editor(s):
Maria-Angeles Aller
Complutense University of Madrid
Spain


Jaime Arias
Complutense University of Madrid
Spain




Contributor(s):
Maria-Angeles Aller
Titular Professor, Department of Surgery I
Medical School, Complutense University of Madrid
Madrid
Spain


Salvador Argudo
General and Digestive Surgeon
Unit of General and Digestive Surgery, Sudeste Hospital, Arganda del Rey
Madrid
Spain


Jaime Arias
Head Professor, Department of Surgery I
Medical School, Complutense University of Madrid
Madrid
Spain


Jorge-Luis Arias
Head Professor, Neurosciences Unit, Department of Psychobiology
School of Psychology, University of Oviedo
Asturias
Spain


Jose-Ignacio Arias
Chairmen, General and Digestive Surgery Unit, Monte Naranco Hospital, Oviedo
Asturias
Spain


Arturo Cruz
General and Digestive Surgeon
Madrid
Spain


Diana Curras
Predoctoral Researcher, Department of Surgery I
Medical School, Complutense University of Madrid
Madrid, 100083
Spain


Jose García-Domínguez
Plastic and Reconstructive Surgeon
Unit of Plastic and Reconstructive Surgery. Getafe Hospital. Getafe
Madrid
Spain


Laudino Lopez
Titular Professor, Neurosciences Unit, Department of Psychobiology
School of Psychobiology, University of Oviedo
Asturias
Spain


Laureano Lorente
Titular Professor, Department of Surgery I
Medical School, Complutense University of Madrid
Madrid
Spain


Manuel Losada
General and Digestive Surgery
Unit of General and Digestive Surgery, Sudeste Hospital, Arganda del Rey
Madrid
Spain


Marta Méndez
Assistant Professor, Neuroscience Unit, Department of Psychobiology
School of Psychobiology, University of Oviedo
Asturias
Spain


Maria-Paz Nava
Titular Professor, Department of Animal Physiology (Physiology II)
Biology School, Complutense University of Madrid
Madrid
Spain


Ana de Paz
Predoctoral Researcher, Department of Surgery I
Medical School, Complutense University of Madrid
Madrid
Spain


Isabel Prieto
General and Digestive Surgeon, Department of Surgery
La Paz Hospital, Autonoma University of Madrid
Madrid
Spain


Fernando Sánchez-Patán
Postdoctoral Researcher, Department of Surgery I
Medical School, Complutense University of Madrid
Madrid
Spain


Patri Vergara
Head Professor, Department of Physiology
School of Veterinary, Autonoma University of Barcelona
Barcelona
Spain




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