Antibodies Applications and New Developments


Eline P. Meulenberg

DOI: 10.2174/97816080526461120101
eISBN: 978-1-60805-264-6, 2012
ISBN: 978-1-60805-586-9

Indexed in: Scopus, Book Citation Index, Science (BKCI-S), Web of Science, BIOSIS Previews, EBSCO.

Antibodies Applications and New Developments is an overview of the current developments of techniques and methods rel...[view complete introduction]
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Principles and Applications of Immunoaffinity Chromatography

- Pp. 156-174 (19)

Abby J. Jackson, Matthew R. Sobansky and David S. Hage


Immunoaffinity Chromatography (IAC) is a type of liquid chromatography that uses the highly specific binding of antibodies for the retention of a target. This approach can be used in a myriad of techniques for the selective purification, concentration and/or analysis of target compounds. This chapter discusses the basic components of an IAC method, including the support, stationary phase, immobilization technique, and proper sample application and elution conditions. Several applications of IAC are also considered. These applications include immunoextraction, immunodepletion, chromatographic immunoassays, and post-column immunodetection. A wide range of targets that have been isolated or measured by these techniques are listed. Related methods based on antibody mimics are also discussed.

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